TLT 212 | Course Introduction and Application Information

Course Name
Histological Techniques
Code
Semester
Theory
(hour/week)
Application/Lab
(hour/week)
Local Credits
ECTS
TLT 212
Fall/Spring
2
2
3
4

Prerequisites
None
Course Language
Turkish
Course Type
Elective
Course Level
Short Cycle
Course Coordinator
Course Lecturer(s)
Assistant(s) -
Course Objectives It is aimed to learn how to prepare the most used slides for light microscopy in order to examine cells and tissues histologically.
Course Description The students who succeeded in this course;
  • Describe the steps of the parafine tissue attachment protocol
  • Describe the names of the most commonly used fixative, dehydration and clarifying agents and their mechanisms of action during tissue examination
  • Describe the cause of the three problems and the solution to the problems encountered during sectioning
  • Describe the most commonly used staining method in histochemistry and which cell parts are stained
  • Explain three specific histochemical stains for use
Course Content Histochemical procedures, paraffin tissue tracing, sectioning and staining procedures will be performed to evaluate these derste cells and tissues under light microscope. It is aimed that the students will be able to transfer the general histology knowledge of the four basic tissues to the concepts and applications of the theoretical knowledge they learn.

 



Course Category

Core Courses
Major Area Courses
X
Supportive Courses
Media and Management Skills Courses
Transferable Skill Courses

 

WEEKLY SUBJECTS AND RELATED PREPARATION STUDIES

Week Subjects Related Preparation
1 Introduction to the course, introduction of laboratories Microscopy, Wet Multidisciplinary Lab Processing Histolojik Boyama Teknikleri Başvuru Kitabı. Editor: Ramazan Demir, Ankara 2001, Palme Publishing, 1-2
2 Acquisition of Tissues Fixation Histolojik Boyama Teknikleri Başvuru Kitabı. Editor: Ramazan Demir, Ankara 2001, Palme Publishing, 3-14
3 Paraffin Tissue Track Dehydration, Transparency Histolojik Boyama Teknikleri Başvuru Kitabı. Editor: Ramazan Demir, Ankara 2001, Palme Publishing, 22-24
4 Paraffin Tissue Track Paraffined Embedding Histolojik Boyama Teknikleri Başvuru Kitabı. Editor: Ramazan Demir, Ankara 2001, Palme Publishing, 24-31
5 Epithelial Tissue- Microscopy Practice Ross MH, Pawlina W, Histoloji Konu Anlatımı ve Atlas, İlişkili Hücre Biyolojisi ve Moleküler Biyoloji ile, Translation of 6. Publish, Translation Editor: Barış Baykal, 2014, Palme Publishing, 152-156
6 Connective Tissue - Microscopy Practice Ross MH, Pawlina W, Histoloji Konu Anlatımı ve Atlas, İlişkili Hücre Biyolojisi ve Moleküler Biyoloji ile, Translation of 6. Publish, Translation Editor: Barış Baykal, 2014, Palme Publishing, 192-196
7 Midterm
8 Cartilage and Bone Tissue - Microscopy Practice Ross MH, Pawlina W, Histoloji Konu Anlatımı ve Atlas, İlişkili Hücre Biyolojisi ve Moleküler Biyoloji ile, Translation of 6. Publish, Translation Editor: Barış Baykal, 2014, Palme Publishing, 244-252
9 Muscular Tissue- Microscopy Practice Ross MH, Pawlina W, Histoloji Konu Anlatımı ve Atlas, İlişkili Hücre Biyolojisi ve Moleküler Biyoloji ile, Translation of 6. Publish, Translation Editor: Barış Baykal, 2014, Palme Publishing, 340-350
10 Nervous System - Microscopy Practice Ross MH, Pawlina W, Histoloji Konu Anlatımı ve Atlas, İlişkili Hücre Biyolojisi ve Moleküler Biyoloji ile, Translation of 6. Publish, Translation Editor: Barış Baykal, 2014, Palme Publishing, 390-398
11 Sectioning, histological staining Haematoxylene-Eosin Staining Histolojik Boyama Teknikleri Başvuru Kitabı. Editor: Ramazan Demir, Ankara 2001, Palme Publishing, 31-34-51
12 Histological Stainings-Specific Connective Tissue Staining Histolojik Boyama Teknikleri Başvuru Kitabı. Editor: Ramazan Demir, Ankara 2001, Palme Publishing, 70-74.
13 Histological Stainings Immunohistochemical staining Histolojik Boyama Teknikleri Başvuru Kitabı. Editor: Ramazan Demir, Ankara 2001, Palme Publishing, 285-289
14 Histological Stainings Immunohistochemical staining Histolojik Boyama Teknikleri Başvuru Kitabı. Editor: Ramazan Demir, Ankara 2001, Palme Publishing, 290-291
15 Review of the Semester
16 Final exam

 

Course Notes/Textbooks

Histolojik Boyama Teknikleri Başvuru Kitabı. Editor: Ramazan Demir, Ankara 2001, Palme Publishing

Ross MH, Pawlina W, Histoloji Konu Anlatımı ve Atlas, İlişkili Hücre Biyolojisi ve Moleküler Biyoloji ile, Translation of 6. Publish, Translation Editor: Barış Baykal, 2014, Palme Publishing

Suggested Readings/Materials

 

EVALUATION SYSTEM

Semester Activities Number Weigthing
Participation
Laboratory / Application
1
20
Field Work
Quizzes / Studio Critiques
5
10
Homework / Assignments
5
15
Presentation / Jury
Project
Seminar / Workshop
Oral Exams
Midterm
1
25
Final Exam
1
30
Total

Weighting of Semester Activities on the Final Grade
70
Weighting of End-of-Semester Activities on the Final Grade
30
Total

ECTS / WORKLOAD TABLE

Semester Activities Number Duration (Hours) Workload
Theoretical Course Hours
(Including exam week: 16 x total hours)
16
2
32
Laboratory / Application Hours
(Including exam week: 16 x total hours)
16
2
Study Hours Out of Class
16
1
Field Work
Quizzes / Studio Critiques
5
2
Homework / Assignments
5
1
Presentation / Jury
Project
Seminar / Workshop
Oral Exam
Midterms
1
5
Final Exam
1
10
    Total
110

 

COURSE LEARNING OUTCOMES AND PROGRAM QUALIFICATIONS RELATIONSHIP

#
Program Competencies/Outcomes
* Contribution Level
1
2
3
4
5
1

 To have the required contemporary theoretical and practical knowledge in his/her field

X
2

To use the material and technology related to his/her field, and make their maintenance, use the information and communication technologies at basic level

X
3

To have the competency to recognize the problems in his/her field, analyze them, develop evidence-based solutions and have the ability to share their suggestions with others

X
4

To be aware of legal responsibilities, conduct basic studies in her/his field independently

X
5

To communicate with patients, relatives and colleagues properly, comprehensively, honestly and explicitly, transfer his/her thoughts and knowledge through written and oral communication

6

To take responsibility as an active team member during the practices in his/her field

7

To commentate and evaluate the scientific information with a critical approach by the help of knowledge gained in his/her field

X
8

To comprehend the importance of lifelong learning, to determine and meet her/his learning needs, to develop herself/himself by monitoring the development in science and technology

X
9

To act by considering the universal ethical values, social and cultural characteristics

10

To know the concepts of occupational safety, patient safety, environmental protection and quality, and fulfill the requirements

X
11

To be able to follow information in his field and communicate with colleagues in English at least a level of European Language Portfolio A2 General Level

12

To take necessary measures in accordance with laboratory safety and protection rules in the field of medical laboratory techniques

X
13

To determine the needs according to the requirements of the laboratory processes and carry out activities for development

*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest