TLT 214 | Course Introduction and Application Information

Course Name
Molecular Biological Methods
Code
Semester
Theory
(hour/week)
Application/Lab
(hour/week)
Local Credits
ECTS
TLT 214
Fall/Spring
3
0
3
4

Prerequisites
None
Course Language
Turkish
Course Type
Elective
Course Level
Short Cycle
Course Coordinator
Course Lecturer(s)
Assistant(s) -
Course Objectives Molecular biological methods are widely used for diagnosis and follow-up in medical laboratories. The aim of this course is to learn the working principles of molecular biological methods used in medical laboratories.
Course Description The students who succeeded in this course;
  • Describe working principles in molecular biology laboratories
  • Explain DNA isolation
  • Explain RNA isolation
  • Describe the methods of polymerase chain reaction
  • Explain agarose gel electrophoresis
  • Classify microarray and DNA sequencing methods
Course Content This course includes introduction to the general tools and materials used in molecular biology laboratory, working rules in molecular biology laboratories, isolation and amplification of DNA and RNA from blood and tissues, visualization and interpretation of amplified products in agarose gel electrophoresis, types of polymerase chain reaction, blotting, RFLP, strip test analysis, microarray and DNA sequencing methods.

 



Course Category

Core Courses
Major Area Courses
X
Supportive Courses
Media and Management Skills Courses
Transferable Skill Courses

 

WEEKLY SUBJECTS AND RELATED PREPARATION STUDIES

Week Subjects Related Preparation
1 Introduction to the course, Introduction and tools used in Molecular Biology Laboratories Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 237-238.
2 Issues to be considered in Molecular Biology Laboratories and Contamination Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 242-246.
3 Isolation of DNA From Blood, Isolation of DNA From Tissues Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 239-240.
4 Isolation of DNA From Amniotic Fluid And Paraffin-Embedded Tissue, Spectrophotometric Determination of the Amount of DNA. Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 241-244.
5 Polymerase Chain Reaction (PCR), Multiplex and Real Time PCR Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 250-258.
6 Preparation of Polymerase Chain Reaction Mix, Mix Dispersion and Amplification with PCR. Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 250-258.
7 Preparation of Agarose Gel, Appliquing of PCR Products on Agarose Gel, Interpretation of Results, Acrylamide Gel Electrophoresis Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 250-258.
8 Midterm Exam
9 RNA Isolation and cDNA Handling, Reverse Transcriptase-PCR. Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 250-258.
10 RFLP, Preparation of RFLP Mixture, Mix Distribution and Cutting Start Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 265-269.
11 Apply RFLP Product to Applique and Transfer To Nitrocellulose Filter, Staining Nitrocellulose Filter. Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 265-269.
12 Blotting Methods Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 290-296.
13 Strip test analysis Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 290-296.
14 Microarray Technology and DNA Sequencing Analysis Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 299-305.
15 General review
16 Final exam

 

Course Notes/Textbooks

Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri.

Suggested Readings/Materials

 

EVALUATION SYSTEM

Semester Activities Number Weigthing
Participation
1
10
Laboratory / Application
Field Work
Quizzes / Studio Critiques
2
10
Homework / Assignments
1
10
Presentation / Jury
Project
Seminar / Workshop
Oral Exams
Midterm
1
30
Final Exam
1
40
Total

Weighting of Semester Activities on the Final Grade
60
Weighting of End-of-Semester Activities on the Final Grade
40
Total

ECTS / WORKLOAD TABLE

Semester Activities Number Duration (Hours) Workload
Theoretical Course Hours
(Including exam week: 16 x total hours)
16
3
48
Laboratory / Application Hours
(Including exam week: 16 x total hours)
16
Study Hours Out of Class
15
1
Field Work
Quizzes / Studio Critiques
2
4
Homework / Assignments
1
4
Presentation / Jury
Project
Seminar / Workshop
Oral Exam
Midterms
1
14
Final Exam
1
16
    Total
105

 

COURSE LEARNING OUTCOMES AND PROGRAM QUALIFICATIONS RELATIONSHIP

#
Program Competencies/Outcomes
* Contribution Level
1
2
3
4
5
1

 To have the required contemporary theoretical and practical knowledge in his/her field

X
2

To use the material and technology related to his/her field, and make their maintenance, use the information and communication technologies at basic level

X
3

To have the competency to recognize the problems in his/her field, analyze them, develop evidence-based solutions and have the ability to share their suggestions with others

X
4

To be aware of legal responsibilities, conduct basic studies in her/his field independently

X
5

To communicate with patients, relatives and colleagues properly, comprehensively, honestly and explicitly, transfer his/her thoughts and knowledge through written and oral communication

6

To take responsibility as an active team member during the practices in his/her field

7

To commentate and evaluate the scientific information with a critical approach by the help of knowledge gained in his/her field

X
8

To comprehend the importance of lifelong learning, to determine and meet her/his learning needs, to develop herself/himself by monitoring the development in science and technology

X
9

To act by considering the universal ethical values, social and cultural characteristics

10

To know the concepts of occupational safety, patient safety, environmental protection and quality, and fulfill the requirements

X
11

To be able to follow information in his field and communicate with colleagues in English at least a level of European Language Portfolio A2 General Level

12

To take necessary measures in accordance with laboratory safety and protection rules in the field of medical laboratory techniques

X
13

To determine the needs according to the requirements of the laboratory processes and carry out activities for development

*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest