VOCATIONAL SCHOOL OF HEALTH SERVICES

Department of Medical Laboratory Techniques

TLT 214 | Course Introduction and Application Information

Course Name
Molecular Biological Methods
Code
Semester
Theory
(hour/week)
Application/Lab
(hour/week)
Local Credits
ECTS
TLT 214
Fall/Spring
3
0
3
4

Prerequisites
None
Course Language
Turkish
Course Type
Elective
Course Level
Short Cycle
Mode of Delivery face to face
Teaching Methods and Techniques of the Course Discussion
Case Study
Simulation
Lecture / Presentation
Course Coordinator
Course Lecturer(s)
Assistant(s) -
Course Objectives Molecular biological methods are widely used for diagnosis and follow-up in medical laboratories. The aim of this course is to learn the working principles of molecular biological methods used in medical laboratories.
Learning Outcomes The students who succeeded in this course;
  • Define the functioning of molecular biology laboratories, protection rules, devices and materials used.
  • Explain DNA and RNA isolation methods.
  • Define the basic principle of polymerase chain reaction (PZT) and compare the intended use of current PZT types.
  • Explain the application steps of agarose gel electrophoresis and the problems encountered.
  • Discuss the application areas of restriction enzymes, DNA sequence analysis methods and the results of analysis.
  • Compare blotting methods.
Course Description This course includes an introduction to the general tools and materials used in a molecular biology laboratory, working rules in molecular biology laboratories, DNA and RNA isolation methods, polymerase chain reaction and its types, visualization and interpretation of amplified products in agarose gel electrophoresis, restriction enzymes and their applications, blotting techniques, microarray, DNA sequencing methods, and basic cell culture techniques.

 



Course Category

Core Courses
Major Area Courses
X
Supportive Courses
Media and Management Skills Courses
Transferable Skill Courses

 

WEEKLY SUBJECTS AND RELATED PREPARATION STUDIES

Week Subjects Related Preparation
1 Introduction to the course, Introduction and tools used in Molecular Biology Laboratories Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 237-238.
2 Issues to be considered in Molecular Biology Laboratories and Contamination Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 242-246.
3 DNA isolation methods - 1 Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 239-240.
4 DNA isolation methods - 2 Spectrophotometric Determination of the Amount of DNA. Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 241-244.
5 Midterm Exam-1
6 Polymerase Chain Reaction (PCR) and Agarose Gel Electrophoresis Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 250-258.
7 RNA Isolation, cDNA Synthesis and Reverse Transcriptase-PCR. Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 250-258.
8 Multiplex and Real Time PCR Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 250-258.
9 Restriction Enzymes and Applications Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 265-269.
10 Midterm Exam-2
11 Microarray Technology and DNA Sequencing Analysis Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 299-305.
12 Blotting Methods (Northern and Southern blots) Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 265-269.
13 Blotting Methods (Western blot) Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 290-296.
14 Cell Culture Techniques Görmüş, U., (2015) Laboratuvar Dünyası Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı, Nobel Tıp Kitabevleri, 290-296.
15 General review
16 Final exam

 

Course Notes/Textbooks

Doç. Dr. Uzay Görmüş, Yard. Doç. Dr. Neşe İnan, Yard. Doç. Dr. İbrahim Söğüt, Dr. Özlem Timirci Kahraman. Laboratuvar Dünyası: Biyokimya, Mikrobiyoloji ve Moleküler Genetik Uygulamalarının Klinik Laboratuvarlarda Kullanımı. Nobel Tıp Kitabevi, 2014, ISBN: 978-605-335-102-3

Suggested Readings/Materials

 

EVALUATION SYSTEM

Semester Activities Number Weigthing
Participation
1
10
Laboratory / Application
Field Work
Quizzes / Studio Critiques
Portfolio
Homework / Assignments
1
20
Presentation / Jury
Project
Seminar / Workshop
Oral Exams
Midterm
2
30
Final Exam
1
40
Total

Weighting of Semester Activities on the Final Grade
4
60
Weighting of End-of-Semester Activities on the Final Grade
1
40
Total

ECTS / WORKLOAD TABLE

Semester Activities Number Duration (Hours) Workload
Theoretical Course Hours
(Including exam week: 16 x total hours)
16
3
48
Laboratory / Application Hours
(Including exam week: '.16.' x total hours)
16
0
Study Hours Out of Class
14
2
28
Field Work
0
Quizzes / Studio Critiques
0
Portfolio
0
Homework / Assignments
2
5
10
Presentation / Jury
0
Project
0
Seminar / Workshop
0
Oral Exam
0
Midterms
2
10
20
Final Exam
1
14
14
    Total
120

 

COURSE LEARNING OUTCOMES AND PROGRAM QUALIFICATIONS RELATIONSHIP

#
Program Competencies/Outcomes
* Contribution Level
1
2
3
4
5
1

To have the required contemporary theoretical and practical knowledge in his/her field

X
2

To use the material and technology related to his/her field, and make their maintenance, use the information and communication technologies at basic level

X
3

To have the competency to recognize the problems in his/her field, analyze them, develop evidence-based solutions and have the ability to share their suggestions with others

4

To be aware of legal responsibilities, conduct basic studies in her/his field independently

5

To communicate with patients, relatives and colleagues properly, comprehensively, honestly and explicitly, transfer his/her thoughts and knowledge through written and oral communication

6

To take responsibility as an active team member during the practices in his/her field

7

To commentate and evaluate the scientific information with a critical approach by the help of knowledge gained in his/her field

X
8

To comprehend the importance of lifelong learning, to determine and meet her/his learning needs, to develop herself/himself by monitoring the development in science and technology

9

To act by considering the universal ethical values, social and cultural characteristics

10

To know the concepts of occupational safety, patient safety, environmental protection and quality, and fulfill the requirements

11

To be able to follow information in his field and communicate with colleagues in English at least a level of European Language Portfolio A2 General Level

12

To take necessary measures in accordance with laboratory safety and protection rules in the field of medical laboratory techniques

13

To determine the needs according to the requirements of the laboratory processes and carry out activities for development

*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest

 


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