VOCATIONAL SCHOOL OF HEALTH SERVICES

Department of Medical Laboratory Techniques

TLT 212 | Course Introduction and Application Information

Course Name
Histological Techniques
Code
Semester
Theory
(hour/week)
Application/Lab
(hour/week)
Local Credits
ECTS
TLT 212
Fall/Spring
2
2
3
4

Prerequisites
None
Course Language
Turkish
Course Type
Elective
Course Level
Short Cycle
Mode of Delivery face to face
Teaching Methods and Techniques of the Course -
Course Coordinator
Course Lecturer(s)
Assistant(s) -
Course Objectives The aim of this course is to learn the most used tissue processing methods in the routine to examine histologically cells and tissues under a light microscope.
Learning Outcomes The students who succeeded in this course;
  • Count the basic steps used in the paraffin tissue processing procedures.
  • Describe the names and acting mechanisms of the most commonly used fixative, dehydration and clarifying agents for tissue processing.
  • Explain the most commonly used staining method in histochemistry and three special histochemical stains and which parts of the cell are stained.
  • Explain the general structure of the cell and functions of the cell organels.
  • Describe the general histological properties of four basic tissues.
  • List the features to be considered while staining.
Course Description In this course, histochemical procedures, paraffin tissue processing, sectioning and staining procedures will be performed to evaluate cells and tissues under a light microscope.

 



Course Category

Core Courses
Major Area Courses
X
Supportive Courses
Media and Management Skills Courses
Transferable Skill Courses

 

WEEKLY SUBJECTS AND RELATED PREPARATION STUDIES

Week Subjects Related Preparation
1 Introduction to the course, introduction of laboratories Microscopy, Wet Multidisciplinary Lab Processing Histolojik Boyama Teknikleri Başvuru Kitabı. Editor: Ramazan Demir, Ankara 2001, Palme Publishing, 1-2
2 Acquisition of Tissues Fixation Histolojik Boyama Teknikleri Başvuru Kitabı. Editor: Ramazan Demir, Ankara 2001, Palme Publishing, 3-14
3 Paraffin Tissue Track Dehydration, Transparency Histolojik Boyama Teknikleri Başvuru Kitabı. Editor: Ramazan Demir, Ankara 2001, Palme Publishing, 22-24
4 Paraffin Tissue Track Paraffined Embedding Histolojik Boyama Teknikleri Başvuru Kitabı. Editor: Ramazan Demir, Ankara 2001, Palme Publishing, 24-31
5 Quiz 1 - Epithelial Tissue- Microscopy Practice Ross MH, Pawlina W, Histoloji Konu Anlatımı ve Atlas, İlişkili Hücre Biyolojisi ve Moleküler Biyoloji ile, Translation of 6. Publish, Translation Editor: Barış Baykal, 2014, Palme Publishing, 152-156
6 Connective Tissue - Microscopy Practice Ross MH, Pawlina W, Histoloji Konu Anlatımı ve Atlas, İlişkili Hücre Biyolojisi ve Moleküler Biyoloji ile, Translation of 6. Publish, Translation Editor: Barış Baykal, 2014, Palme Publishing, 192-196
7 Midterm
8 Cartilage and Bone Tissue - Microscopy Practice Ross MH, Pawlina W, Histoloji Konu Anlatımı ve Atlas, İlişkili Hücre Biyolojisi ve Moleküler Biyoloji ile, Translation of 6. Publish, Translation Editor: Barış Baykal, 2014, Palme Publishing, 244-252
9 Muscular Tissue- Microscopy Practice Ross MH, Pawlina W, Histoloji Konu Anlatımı ve Atlas, İlişkili Hücre Biyolojisi ve Moleküler Biyoloji ile, Translation of 6. Publish, Translation Editor: Barış Baykal, 2014, Palme Publishing, 340-350
10 Nervous System - Microscopy Practice Ross MH, Pawlina W, Histoloji Konu Anlatımı ve Atlas, İlişkili Hücre Biyolojisi ve Moleküler Biyoloji ile, Translation of 6. Publish, Translation Editor: Barış Baykal, 2014, Palme Publishing, 390-398
11 Quiz 2 - Sectioning, histological staining Haematoxylene-Eosin Staining Histolojik Boyama Teknikleri Başvuru Kitabı. Editor: Ramazan Demir, Ankara 2001, Palme Publishing, 31-34-51
12 Histological Stainings-Specific Connective Tissue Staining Histolojik Boyama Teknikleri Başvuru Kitabı. Editor: Ramazan Demir, Ankara 2001, Palme Publishing, 70-74.
13 Histological Stainings Immunohistochemical staining Histolojik Boyama Teknikleri Başvuru Kitabı. Editor: Ramazan Demir, Ankara 2001, Palme Publishing, 285-289
14 Quiz 3 - Histological Stainings Immunohistochemical staining Histolojik Boyama Teknikleri Başvuru Kitabı. Editor: Ramazan Demir, Ankara 2001, Palme Publishing, 290-291
15 Review of the Semester
16 Final exam

 

Course Notes/Textbooks

1. Histolojik Boyama Teknikleri Başvuru Kitabı. Editör: Ramazan Demir, Ankara 2001, Palme Yayıncılık, ISBN : 9786052828618

2. Ross MH, Pawlina W, Histoloji Konu Anlatımı ve Atlas, İlişkili Hücre B,iyolojisi ve Moleküler Biyoloji ile , 6. Baskıdan Çeviri, Çeviri Editörü: Barış Baykal, 2014, Palme Yayıncılık, ISBN: 9786053552239

Suggested Readings/Materials

Junqueira's Temel Histoloji Atlas Kitap, Mescher, 9/2015 , Çeviri Editörü : Seyhun Solakoğlu, Aslı Erdoğan, Hasan Serdar Mutlu, Nobel Tıp Kitabevleri  ISBN: 9786053351429

 

EVALUATION SYSTEM

Semester Activities Number Weigthing
Participation
1
5
Laboratory / Application
Field Work
Quizzes / Studio Critiques
3
15
Portfolio
Homework / Assignments
1
15
Presentation / Jury
Project
Seminar / Workshop
Oral Exams
Midterm
1
25
Final Exam
1
40
Total

Weighting of Semester Activities on the Final Grade
6
60
Weighting of End-of-Semester Activities on the Final Grade
1
40
Total

ECTS / WORKLOAD TABLE

Semester Activities Number Duration (Hours) Workload
Theoretical Course Hours
(Including exam week: 16 x total hours)
16
2
32
Laboratory / Application Hours
(Including exam week: '.16.' x total hours)
16
2
32
Study Hours Out of Class
14
1
14
Field Work
0
Quizzes / Studio Critiques
3
4
12
Portfolio
0
Homework / Assignments
1
8
8
Presentation / Jury
0
Project
0
Seminar / Workshop
0
Oral Exam
0
Midterms
1
10
10
Final Exam
1
12
12
    Total
120

 

COURSE LEARNING OUTCOMES AND PROGRAM QUALIFICATIONS RELATIONSHIP

#
Program Competencies/Outcomes
* Contribution Level
1
2
3
4
5
1

To have the required contemporary theoretical and practical knowledge in his/her field

X
2

To use the material and technology related to his/her field, and make their maintenance, use the information and communication technologies at basic level

X
3

To have the competency to recognize the problems in his/her field, analyze them, develop evidence-based solutions and have the ability to share their suggestions with others

4

To be aware of legal responsibilities, conduct basic studies in her/his field independently

5

To communicate with patients, relatives and colleagues properly, comprehensively, honestly and explicitly, transfer his/her thoughts and knowledge through written and oral communication

6

To take responsibility as an active team member during the practices in his/her field

7

To commentate and evaluate the scientific information with a critical approach by the help of knowledge gained in his/her field

8

To comprehend the importance of lifelong learning, to determine and meet her/his learning needs, to develop herself/himself by monitoring the development in science and technology

9

To act by considering the universal ethical values, social and cultural characteristics

10

To know the concepts of occupational safety, patient safety, environmental protection and quality, and fulfill the requirements

X
11

To be able to follow information in his field and communicate with colleagues in English at least a level of European Language Portfolio A2 General Level

12

To take necessary measures in accordance with laboratory safety and protection rules in the field of medical laboratory techniques

X
13

To determine the needs according to the requirements of the laboratory processes and carry out activities for development

*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest

 


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